Rapid environmental DNA detection with isothermal amplification methods: a comparison with the real-time quantitative PCR in the natterjack toad (Epidalea calamita).
Abigael Chieux, Alessia Guerrieri, Eva Bellemain, Frederic Boyer, Thomas Merzi, Pierre Tablerlet, Amaia Iribar, Lucie Zinger, Aurelie Bonin
This paper compares isothermal amplification methods (LAMP and RPA) with qPCR for detecting natterjack toad eDNA, finding RPA matches qPCR sensitivity while LAMP is less sensitive.
bioRxiv · Nov 9, 2026 · PreprintGenomics