bioRxivEvolutionary Biology
An overview of GabRat edge disruption and its new extensions for unbiased quantification of disruptive camouflaging patterns using randomization technique
Abstract
Disruptive colorations are camouflaging patterns that use contrasting colorations to interrupt the continuously of objects edge and disturb the observers visual recognition. The GabRat method has been introduced and widely used to quantify the strength of edge disruption. The original GabRat method requires a composite image where a target object is placed on a particular background. It computes the intensities of frequency components parallel and perpendicular to the edge direction at each edge point using Gabor filters, and summarize the ratios of these two intensities around the perimeter of the shape. However, we found that the original GabRat method has an issue which produces false signals and biases to overestimating the GabRat value depending on the edge angle. Here, we introduce GabRat-R, which can diminish that angle dependency using Gabor filters with randomized base angles. Additionally, we developed GabRat-RR, which iteratively places a target object on a background with random positions and rotation angles to average the effects of the heterogeneity and anisotropy of background. Compared with the original GabRat, our GabRat-R and GabRat-RR programs run more efficiently using multithreading techniques. GabRat-R and GabRat-RR were freely available in Natsumushi 2.0 software of the authors website.
- assay: imaging
- research focus: cell type annotation
- research focus: clustering
- research focus: dimensionality reduction
- approach: deep learning
- approach: statistical model
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