DevelopmentMolecular BiologyPeer-reviewed journal article
SLAM-ITseq: Sequencing cell type-specific transcriptomes without cell sorting
Wayo MatsushimaiD, Veronika A. Herzog, Tobias NeumanniD, Katharina GappiD, Johannes A. ZuberiD, Stefan Ludwig AmeresiD, Eric Alexander MiskaiD
Abstract
Cell type-specific transcriptome analysis is an essential tool in understanding biological processes but can be challenging due to the limits of microdissection or fluorescence-activated cell sorting (FACS). Here, we report a novel in vivo sequencing method, which captures the transcriptome of a specific type of cells in a tissue without prior cellular or molecular sorting. SLAM-ITseq provides an accurate snapshot of the transcriptional state in vivo.
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