Resilience to multiple stressors in an aquatic plant and its microbiome

Abstract

PremiseEnvironments are changing rapidly, and outcomes of species interactions, especially mutualisms, are notoriously dependent on the environment. A growing number of studies have investigated responses of mutualisms to anthropogenic changes, yet most studies have focused on nutrient pollution or climate change, and tested single stressors. Relatively little is known about impacts of simultaneous chemical contaminants, which may differ fundamentally from nutrient or climate stressors, and are especially widespread in aquatic habitats.\n\nMethodsWe investigated the impacts of two common contaminants on interactions between the common duckweed Lemna minor and its microbiome. Sodium chloride (salt) and benzotriazole (a corrosion inhibitor) negatively affect aquatic organisms individually, yet commonly co-occur in runoff to duckweed-inhabited sites. We tested three L. minor genotypes with and without the culturable portion of their microbiome across field realistic gradients of salt (3 levels) and benzotriazole (4 levels) in a fully factorial experiment (72 treatments), and measured plant and microbial growth.\n\nKey ResultsWe found that stressors had conditional effects. Salt decreased both plant and microbial growth, but decreased plant survival more as benzotriazole concentrations increased. In contrast, benzotriazole did not affect microbial abundance, and benefited plants when salt and microbes were absent, perhaps due to the biotrans-formation we observed without salt. Microbes did not ameliorate duckweed stressors, as microbial inoculation increased plant growth, but not at high salt concentrations.\n\nConclusionsOur results suggest that multistressor effects matter when predicting responses of mutualisms to global change, but that mutualisms may not buffer organisms from stressors.