Discover, rate, and discuss biology preprints.

CaGE alternates guided structure prediction with sequence redesign, fading guidance each cycle, so sequences recover complex catalytic sites unaided; designed nonheme-iron and PLP-dependent enzymes were active.

Across seven pace-controlled outdoor overground speeds, healthy older adults (n=11) showed no higher gross or net walking cost than younger adults (n=16), trending lower instead.

Reward-guided inference-time search over the Proteína-Complexa sequence-structure codesign model yields validated binders across 127 targets, kinases and a free carbohydrate, with codesigned sequences outperforming ProteinMPNN redesign.

BoneGraph combines a SPECTER2-indexed bone corpus, knowledge graph, deterministic physics checks, and user-taught rules in a local five-tab system; oracle passages raise accuracy 42% to 78%.

FLIP2 adds seven protein fitness datasets and 16 splits for enzymes, light-sensitive proteins, and interactions; simple ridge and zero-shot baselines often rival fine-tuned protein language models.

Brain-stiffness-matched pHEMA/GMA hydrogel scaffolds with 40 µm interconnected pores reduced astrocyte encapsulation and iNOS+ macrophage fraction versus stiff solid rods after 4 weeks in rat brain.

A provenance-tracked multi-agent system curated 57 CAR-T patient scRNA-seq studies, generated reviewable hypotheses, and prioritized KLRC1/NKG2A, whose blockade improved CD8+ CAR-T antitumour activity in a Raji xenograft model.

Four miniature lenses sharing one CMOS sensor, with computational stitching, give cellular-resolution imaging over 7.5 × 10 mm^2 at video rate, resolving 3,474 mouse cortical neurons.

Gold-nanorod surface-enhanced Raman spectroscopy with LightGBM separates CAR T from untransduced T cells, tracks antigen-specific activation dynamics, and discriminates sorted CAR+ from CAR- patient T cells.

A prediction-guided FGF21 construct with a longer C-terminus gave less protein than the literature design yet concentrated to 1.4 mg/mL versus 0.7 mg/mL (n = 1).

Stanford diners (n = 116) rated soy and soy-wheat meatballs below beef-based ones on moistness, meatiness, fattiness and tastiness, yet perceived no hardness or chewiness gap despite instrumental differences.

Across nearly one million progeny from 10 species, species best predicts homing drive inheritance, while promoter, parent sex and maternal deposition each explain little of the inheritance variation.

A single-donor iPSC-derived alveolar chip with epithelial, endothelial and macrophage cells models early Mycobacterium tuberculosis infection and shows ATG14-deficient macrophages die more without extra bacterial replication.

MagPiezo pairs 19 nm anti-Piezo1-conjugated ZnMnFe2O4 nanoparticles with a 1 Hz, <40 mT magnetic applicator to generate piconewton torque that gates endogenous Piezo1 channels in human endothelial cells.

An all-atom diffusion model unifying biomolecular structure prediction and sequence-structure generation designs experimentally validated nanobodies, mini-proteins, and linear or cyclic peptides across 26 diverse targets.

A 16-hour continuous iodixanol density gradient workflow isolates lipid nanoparticle protein coronas from human plasma and shows that vitronectin and C-reactive protein coronas impair mRNA delivery in liver cells.

Embedding day-8 cerebral organoids in Geltrex loaded with human brain microvascular endothelial cells, plus VEGF-supplemented mixed media, yields integrated endothelial networks and almost three-fold less apoptosis.

Smartphone-video biomechanics (OpenCap) reproduced four timed function tests (r > 0.98) and separated facioscapulohumeral from myotonic dystrophy better than test times (balanced accuracy 82% vs 50%; 129 participants).

Treating low-confidence AlphaFold structures as noisy data at pLDDT-dependent diffusion times lets a 16.7M-parameter model generate long proteins with higher diversity, designability and novelty than Proteína.

MATLAB toolsets add joint, muscle, synergy, and foot-contact personalization and optimal-control treatment design to OpenSim; one post-stroke case predicted 60% faster walking at equal metabolic cost.