Toward Quantitative Metabarcoding
A. O. Shelton, Z. J. Gold, A. J. Jensen, E. D'Agnese, E. A. Allan, A. Van Cise, R. Gallego, A. Ramon-Laca, M. Garber-Yonts, K. Parsons, R. P. Kelly
This paper develops a method to estimate starting DNA proportions from amplicon-sequence data by modeling the PCR process, improving the quantitative analysis of metabarcoding studies.
bioRxiv · Apr 26, 2022Ecology