Scalable single-cell isoform profiling with sequencing-by-expansion
Christophe H. Georgescu, Ghamdan Al‐Eryani, Allison Brookhart, Jagdeesh Chandrasekar, Stephanie J. Yaung, Megan Rogers-Peckham, Megan Freer, Michael E. Kartje, Houlin Yu, Akanksha Khorgade, Charlotte Yang, Lacey McGee, Kendall Berg, Cynthia Cech, Salka Barrett, , Aziz M. Al’Khafaji
This paper introduces a high-throughput single-cell RNA isoform sequencing method using sequencing-by-expansion (SBX) and the CUDLL algorithm, achieving high accuracy and enabling isoform-level analysis across cell types.
bioRxiv · Jul 28, 2026 · PreprintGenetics