Best Practices for Pre-Processing Large-Scale ATAC-Seq Data: Why Epidemiological Studies Demand Greater Stringency
Philippa M. Wells, Marina Flores-Payan, Barry A. Chioza, Joe Burrage, Ann Babtie, Sarah J. Marzi, Leonard C. Schalkwyk, Emma Louise Dempster, Jonathan S. Mill, Eilis J. Hannon
The paper introduces PIPPA, a pipeline for processing large-scale ATAC-seq data, showing that peak calling performance varies by dataset and recommending parameters for high-confidence chromatin accessibility profiles.
bioRxiv · Oct 27, 2026 · PreprintBioinformatics