Production of homozygous deletion mutants targeting fertilization regulator genes through multiplex genome editing
Ari Yoshimura, Yura Seo, Sora Kobayashi, Tomoko Igawa
The paper develops a method to produce homozygous deletion mutants of fertilization regulator genes in Arabidopsis using CRISPR/Cas9 with multiplex guide RNAs, achieving successful deletions in the T1 generation.
Plant and Cell Physiology · May 29, 2025Peer-reviewedPlant Biology