Sample preparation for direct nanopore based protein identification via tryptic peptide recognition
Carolin Hensel, Carolin Kleber, Ida Suppanz, Atefeh Akbari, Sebastian Gutsch, Tobias Hutzenlaub, Tobias Ensslen, Jan Claas Behrends, Felix von Stetten, Gerhard Mittler, Jan-Niklas Klatt, Andreas W. Schreiber
This paper introduces a sample preparation workflow (SP3) that enables nanopore-based protein identification by decoupling proteolysis from interfering components, demonstrating its effectiveness with tryptic digests of myelin basic protein.
bioRxiv · Oct 23, 2026 · PreprintBiochemistry