Ultrahigh-Throughput Forster Resonance Energy Transfer-Activated Droplet Sorting for Terminator Polymerase Engineering
Hui Zhang, Qingqing Xie, Haoran Xu, Wei Li, Chuang Li, Liang Shen, Peiyi Wang, Xinjiang Yan, Xiang Li, Shiyue Zhou, Shitian Zhuo, Chuanyu Liu, Bo Teng, Yuliang Dong, Mengzhe Shen, , Xun Xu
This paper introduces a dual-channel FRET-based droplet sorting system to enhance terminator polymerase engineering, achieving 30-fold enrichment of active variants in a single round.
bioRxiv · Dec 20, 2025 · PreprintBiochemistry