High throughput single-cell RNA sequencing of intact adult cardiomyocytes and non-myocytes using a split-pool approach
Yang Hu, Rijan Gurung, Stefan Mueller, Erielle Villanueva, Justus Stenzig, Nirmala Rayan, Tuan Danh Anh Luu, Syahfiqah Nur, Brandon Tan, Boxiang Liu, Haojie Yu, Hyungwon Choi, Roger Foo, Matthew Andrew Ackers-Johnson
This paper develops a high-quality, scalable workflow for whole-cell single-cell RNA sequencing of adult cardiomyocytes and non-myocytes using in-cell ligation and split-pool barcoding, improving data quality and throughput.
bioRxiv · Apr 30, 2026 · PreprintCell Biology