Matryoshka: Ratiometric biosensors from a nested 1 cassette of green- and orange-emitting fluorescent proteins
C. Ast, J. Foret, L. Oltrogge, R. De Michele, T. Kleist, C.-H. Ho, W. Frommer
The paper introduces Matryoshka, a platform for creating dual-fluorescent protein biosensors with improved dynamic range and reduced artifacts by nesting a reference FP within a reporter FP.
bioRxiv · Jun 6, 2017Cell Biology