A chaperone-guided donor-strand exchange pathway assembles a Gram-positive pilus
Mike Sleutel, Caleb Anderson, Mélanie Roch, Casandra Tan, Adeline Mei Hui Yong, Claudia Gräf, Deepti Rawat, Caroline S. Manzano, Han K. Remaut, Kimberly A. Kline
This study identifies a chaperone-guided donor-strand exchange pathway in Enterococcus faecalis that assembles a Gram-positive pilus, revealing a hybrid system combining chaperone-usher and sortase-dependent mechanisms.
bioRxiv · Oct 17, 2026 · PreprintMicrobiology