SHARK: a specialized host for assembling R6K plasmids
Shivang Hina-Nilesh Joshi, Christopher Jenkins, David Ulaeto, Thomas E. Gorochowski
The paper introduces SHARK, a series of Pir E. coli strains designed for efficient cloning of R6K plasmids, showing they are >100-fold more efficient than a commercial strain for complex cloning.
ACS Synthetic Biology · Jan 13, 2026Peer-reviewedSynthetic Biology