An E2-ubiquitin thioester-driven approach to identify substrates modified with ubiquitin and ubiquitin-like molecules
G. Bakos, L. Yu, I. A. Gak, T. I. Roumeliotis, D. Liakopoulos, J. S. Choudhary, J. Mansfeld
This paper introduces E2~dID, a method to identify substrates modified by ubiquitin and ubiquitin-like molecules using E2-thioester conjugates, validated in human and yeast systems.
bioRxiv · Oct 10, 2018Biochemistry