Cell-free biosynthesis combined with deep learning accelerates de novo-development of antimicrobial peptides
Amir Pandi, David Adam, Amir Zare, Van Tuan Trinh, Stefan L. Schaefer, Marie Burt, Björn Klabunde, Elizaveta Bobkova, Manish Kushwaha, Yeganeh Foroughijabbari, Peter R. Braun, Christoph K. Spahn, Christian Preußer, Elke Pogge von Strandmann, Helge B. Bode, , Tobias J. Erb
Autoencoder design plus a roughly 24-hour cell-free screen tested 500 candidates over five rounds, finding 30 functional AMPs; six were broad-spectrum with no resistance emerging in E. coli.
Nature Communications · Nov 8, 2023Peer-reviewedSynthetic Biology✦ In-depth digest