PacBio library preparation using blunt-end adapter ligation produces significant artefactual fusion DNA sequences
Paul Griffith, Castle Raley, David Sun, Yongmei Zhao, Zhonghe Sun, Monika Mehta, Bao Quoc Tran, Xiaolin Wu
The paper identifies artefactual fusion DNA sequences produced by blunt-end adapter ligation in PacBio library preparation and recommends an alternative A/T overhang method to reduce them.
bioRxiv · Jan 9, 2018 · PreprintGenetics