Probing the role of residues lining the active site in the generation of glucose-tolerant variants of a fungal GH1 enzyme
Barnava Banerjee, Dipayan Chatterjee, Pritha Dasgupta, Chinmay K. Kamale, Prasenjit Bhaumik
This study identifies glucose-tolerant variants of a fungal β-glucosidase through site-specific mutations, improving its industrial applicability by enhancing glucose tolerance and kinetic properties.
bioRxiv · Mar 11, 2026 · PreprintBiochemistry