Structural and Functional Comparison of SARS-CoV-2-Spike Receptor Binding Domain Produced in Pichia pastoris and Mammalian Cells
Argentinian AntiCovid Consortium, C. R. Arbeitman, G. Auge, M. Blaustein, L. Bredeston, E. S. Corapi, P. O. Craig, L. A. Cossio, L. Dain, C. D'Alessio, F. Elias, N. B. Fernandez, J. Gasulla, N. Gorojovsky, G. E. Gudesblat, , A. M. Zelada
This study compares the structural and functional properties of SARS-CoV-2 Spike RBD produced in yeast and mammalian cells, finding similar stability but greater heterogeneity in yeast-produced RBD.
bioRxiv · Sep 17, 2020 · PreprintBiochemistry