CascadeMAP: Autonomous Closed-loop Optimization of Enzyme Cascades via Microfluidics, Machine Learning and Agentic AI
Michal Vasina, David R. Kovar, Martin Kizovský, David Lacko, Pavel Vaňáček, Maximilian Herich, Eduard Volf, Lukas Drdla, Soňa Cabalová, Pavlína Sikorová, Michael Jirásek, Pavel Solanský, Jan Ježek, Ota Samek, Filip Dousek, , Zbyněk Prokop
Unattended droplet-microfluidic Bayesian optimization of a glycerol detection cascade matched design of experiments (DoE) with one third of the conditions; a second cascade used DoE with Raman readout.
bioRxiv · Oct 21, 2026 · PreprintBiochemistry✦ In-depth digest