HiSpoT: a novel one-tube dual-barcode PCR method for NGS spoligotyping amplicon library preparation in Mycobacterium tuberculosis complex
Paul Jeffrey Freidlin, Mor Rubinstein, Ephraim Fass, Inna Kutikov, Gal Zizelski Valenci, Yelena Losev, Zeev Dveyrin, Efrat Rorman, Israel Nissan
HiSpoT is a PCR-based method for preparing NGS libraries for spoligotyping MTBC isolates, offering high-throughput, low-cost, and accurate strain typing with open-source tools for analysis.
bioRxiv · Nov 5, 2026 · PreprintMolecular Biology