Decoupling the bridge helix of Cas12a results in a reduced trimming activity and impaired conformational transitions
E. Woerle, L. Jakob, A. Schmidbauer, G. Zinner, D. Grohmann
This study shows that modifying the bridge helix of Cas12a reduces its trimming activity and affects its conformational changes.
bioRxiv · Feb 25, 2021Biochemistry