Evaluating the reliability of tools for mRNA annotation and IRES studies
Gemma May, Christina Akirtava, C. Joel McManus
A back-splicing circRNA reporter yields linear trans-spliced artifacts; in orthogonal assays three reported cellular IRESes are inactive, and Iso-Seq 5' ends agree with nAnT-iCAGE start sites.
bioRxiv · Sep 25, 2026 · PreprintGenomics✦ In-depth digest