A portable C-terminal rheostat calibrates RecE/Redα exonuclease activity for enhanced bacterial recombineering
Ji Luan, Chanjuan Jiang, Ruofei Guo, Chongyang Wang, Shaoqian Wang, A. Francis Stewart, Jun Fu, Youming Zhang, Hailong Wang
The paper identifies a highly active RecET recombinase pair for efficient homologous recombination in Streptomyces, enabling large DNA integration with minimal homology arms.
bioRxiv · Oct 30, 2026 · PreprintMolecular Biology