NEK1 autophosphorylation is disrupted by amyotrophic lateral sclerosis-associated missense variants: activity biomarkers and structural insights
Shalini Agarwal, S.A. Abdul Rehman, Ivan M. Munoz, Axel Knebel, Paul J. Hop, Robert Gourlay, Fiona Brown, Thomas Macartney, Iolo Squires, Jan Herman Veldink, Kevin Patrick Kenna, John Rouse, Arpan R. Mehta
Kinase-dead controls, XIC quantification and phosphospecific antibodies establish pSer14, pThr156 and pSer418 as NEK1 autophosphorylation sites; a pThr156 assay shows ALS missense variants differ, with R261C reducing it most.
Open Biology · Oct 7, 2026Peer-reviewedNeuroscience✦ In-depth digest