Author

Johannes Stein

Every paper listing this name carries an ORCID.

Identified by ORCID

Johannes SteiniD3 papers · Harvard University

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Assay:fluorescence / live-cell microscopy· 2 Approach:Bayesian inference· 1 Research Focus:cell type annotation· 1 Assay:imaging· 1 Research Focus:imputation· 1 Approach:microscopy & imaging analysis· 1

Frequent Co-Authors

George McDonald ChurchiD· 2 shared Alexander V. HillsleyiD· 1 shared David L. SterniD· 1 shared Jan FunkeiD· 1 shared Lorenzo MagniiD· 1 shared Paul W. TillbergiD· 1 shared Rebecca T. PerelmaniD· 1 shared

Activity by Year

2025-2025 · peak 3/yr

Co-Author Collaboration Network

7 top collaborators · 8 mutual co-author links

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2 shared papersBiophysics

George McDonald ChurchORCID

Mutual co-authors in cluster

Author profile →

3 papers

Recent·Most cited

A simple, ultrastable, and cost-effective oxygen-scavenging system for long-term DNA-PAINT imaging

Rebecca T. Perelman, George McDonald Church, Johannes Stein

This paper introduces an enzyme-free oxygen-scavenging buffer for DNA-PAINT imaging that improves stability, reduces costs, and extends imaging duration.

Small · Dec 19, 2025Peer-reviewedBiophysics

DNA-PAINT imaging with hydrogel imprinting and clearing

Johannes Stein, Lorenzo Magni, George McDonald Church

This study shows that polyacrylamide hydrogels support DNA-PAINT imaging by preserving nanoscale docking strand positioning and enhancing diffusion and accessibility.

ACS Sensors · May 9, 2025Peer-reviewedBiophysics

A Bayesian Solution to Count the Number of Molecules within a Diffraction Limited Spot

Alexander V. Hillsley, Johannes Stein, Paul W. Tillberg, David L. Stern, Jan Funke

This paper introduces a Bayesian method to estimate the number of fluorescent emitters in diffraction-limited spots, showing improved accuracy over existing methods.

Nano Letters · Apr 4, 2025Peer-reviewedBiophysics