Structural models of mitochondrial uncoupling proteins obtained in DPC micelles are not physiologically relevant for their uncoupling activity.
M. Piel, S. Masscheleyn, F. Bouillaud, K. Moncoq, B. MIROUX
The paper shows that UCP1 mutants identified through NMR in DPC detergent do not affect activation in a yeast mitochondrial assay, suggesting DPC-based models are not physiologically relevant.
bioRxiv · Jul 20, 2020Biochemistry