High-throughput Single-cell Proteomics Enabled by an Integrated Hyperplexing and Automatic Labelling Approach without Fractionation
Hui Zhang, Qing Zeng, Kai Jin, Chuanxi Huang, Xing Liu, Jing Yang, Hanbin Ma, Fuchu He, Yun Yang
This paper introduces Integral-Hyperplex, a method combining isobaric tags and tandem mass tags to increase single-cell proteomics throughput by 30-fold, validated through quantification experiments and automated labeling.
bioRxiv · Jun 22, 2025 · PreprintBiochemistry