Excessive sample reagent incubation time increases the risk of false rifampin resistance results in Xpert(R) MTB/RIF Ultra tests performed on paucibacillary Mycobacterium tuberculosis sputum.
Gayatri Shankar Chilambi, Kunj Modi, Paulami Rudra, Yiwang Chen, Soumitesh Chakravorty, David Alland
This study shows that extended incubation of paucibacillary sputum samples beyond 4 hours in sample reagent increases false rifampin resistance results in Xpert MTB/RIF Ultra tests.
bioRxiv · Oct 13, 2026 · PreprintMicrobiology