Use of a counterselectable transposon to create markerless knockouts from a 18,432-clone ordered M. bovis BCG mutant resource
K. Borgers, K. Vandewalle, A. Van Hecke, G. Michielsen, E. Plets, L. van Schie, S. Vanmarcke, L. Schindfessel, N. Festjens, N. Callewaert
The paper describes a method to generate markerless knockouts from a large transposon mutant library in M. bovis BCG, creating an ordered mutant resource targeting 83% of non-essential genes.
bioRxiv · Oct 24, 2019 · PreprintMicrobiology