Deep mutational scanning by FACS-sorting of encapsulated E. coli micro-colonies
Lars Behrendt, Amelie Stein, Shiraz Ali Shah, Karsten Zengler, Søren J. Sørensen, Kresten Lindorff-Larsen, Jakob R. Winther
This paper introduces a method for high-throughput screening of protein variants using encapsulated E. coli micro-colonies and evaluates biophysical predictors for mutation effects on GFP fluorescence.
bioRxiv · Mar 2, 2018 · PreprintBioengineering