In vivo delivery of functional Cas:DNA nucleoprotein complexes into recipient bacteria through a Type IV Secretion System
D. L. Guzman-Herrador, A. Fernandez-Gomez, F. Depardieu, D. Bikard, M. Llosa
This paper demonstrates the delivery of functional Cas:DNA nucleoprotein complexes into recipient bacteria via a Type IV Secretion System, enabling targeted genetic modification without requiring nuclease expression in target cells.
bioRxiv · Jan 18, 2024Microbiology