Improvement of thermostability and activity of PET-degrading enzyme Cut190 towards a detailed understanding and application of the enzymatic reaction mechanism
The paper improves the thermostability and activity of the PET-degrading enzyme Cut190 through mutagenesis and structural analysis, revealing the role of Ca2+ ions and a novel ejecting form in the reaction mechanism.
bioRxiv · Feb 26, 2023 · PreprintBiochemistry