Ancestral Protein Reconstruction of a membrane trafficking GTPase uncovers unanticipated properties of the ancestral protein and of modern Arf1 GTPases
Marine Alves, Mandeep Sivia, Kristína Záhonová, Catherine L. Jackson, Joel B. Dacks
This study reconstructs an ancestral GTPase and finds it localized to both Golgi and plasma membrane, a feature retained in modern Arf1 proteins.
bioRxiv · Oct 27, 2025 · PreprintEvolutionary Biology