Negative epistasis limits current codon optimization approaches
Marco Gees, Zrinka Raguz Nakic, Maria O. Anisimova, Víctor García, Christin Peters
The paper evaluates codon optimization approaches for protein expression in E. coli, finding that negative epistasis limits prediction accuracy and that non-proprietary methods can achieve high expression.
bioRxiv · Jun 3, 2025 · PreprintMolecular Biology