Developmental BiologyDevelopmental Biology AdvancesPeer-reviewed
Gene editing without a genome: generation and validation of F0 CRISPR mutants in gastropod mollusc Crepidula fornicata
Danielle C. Jordan, Molly L. Rivers, Lucy Belmont…·Victoria A. Sleight
The paper generates and validates F0 CRISPR-Cas9 pax6 knockout mutants in the gastropod Crepidula fornicata without an assembled genome, showing eye-loss phenotype and confirming edits via Sanger sequencing and ICE analysis.
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