High-Throughput Global Phosphoproteomic Profiling Using Phospho Heavy-Labeled-Spiketide FAIMS Stepped-CV DDA (pHASED)
D. E. Staudt, H. C. Murray, D. A. Skerrett-Byrne, N. D. Smith, M. F. Jamaluddin, R. G. S. Kahl, R. J. Duchatel, Z. Germon, T. McLachlan, E. R. Jackson, I. J. Findlay, P. S. Kearney, A. Mannan, H. P. McEwen, A. M. Douglas, , M. D. Dun
This paper introduces a new phosphoproteomics protocol, pHASED, which reduces sample preparation time and increases phosphoproteomic coverage compared to traditional methods, revealing potential therapeutic targets in AML.
bioRxiv · Apr 22, 2022 · PreprintBiochemistry