Computational Design and Atomistic Validation of a High-Affinity VHH Nanobody Targeting the PI/RuvC Interface of Streptococcus pyogenes Cas9: A Bivalent Hub Strategy for CRISPR-Cas9 Enhancement
N. Vinay Kumar, Dinky Dalal, Vishakha Sharma
The paper computationally designs a VHH nanobody targeting Cas9's PI/RuvC interface and validates its structure and stability through molecular dynamics simulations.
bioRxiv · Mar 25, 2026 · PreprintBioinformatics