An isothermal calorimetry assay for determining steady state kinetic and enzyme inhibition parameters for SARS-CoV-2 3CL-protease
L. Mazzei, S. Ranieri, R. Greene-Cramer, C. Cioffi, G. T. Montelione, S. Ciurli
This paper develops an ITC assay to measure kinetic and inhibition parameters of SARS-CoV-2 3CL-protease, revealing two inhibition modes and deriving kinetic constants with high precision.
bioRxiv · Jan 31, 2024 · PreprintBiochemistry