Mechanisms involved in the lipid membrane tubulation activity of the pH-responsive MakA protein from Vibrio cholerae
Athar Alam, Abdelbasset Yabrag, Naeem Ullah, Rasmus Sten, Jörgen Ådén, Nandita Bodra, Hudson P. Pace, Marta Bally, Karina Persson, Bernt Eric Uhlin, Sun Nyunt Wai, Aftab Nadeem
This study identifies the molecular mechanisms of MakA's pH-dependent lipid membrane tubulation, showing that His30 protonation and cholesterol binding drive conformational changes necessary for tubulation.
bioRxiv · Dec 8, 2025 · PreprintBiochemistry