Protein content and lipid profiling of isolated native autophagosomes
A. Kern, D. Schmitt, S. Bozkurt, P. Henning-Domres, H. Huesmann, S. Eimer, L. Bindila, G. Tascher, C. Münch, C. Behl
The paper isolates native autophagosomes using FACS and profiles their protein content and phospholipid composition under different autophagy conditions, finding that proteasome inhibition affects cargo more than starvation.
bioRxiv · Apr 16, 2021 · PreprintBiochemistry