Microbial community composition and diversity via 16S rRNA gene amplicons: evaluating the Illumina platform
Lucas Sinclair, Omneya Ahmed Osman, Stefan Bertilsson, Alexander Eiler
The paper evaluates the reliability and biases of Illumina MiSeq platform for 16S rRNA gene amplicon sequencing, finding similar performance to 454 pyrosequencing but with notable differences in evenness estimates.
bioRxiv · Oct 6, 2014Molecular Biology