Evolving a mitigation of the stress response pathway to change the basic chemistry of life
Isabella Tolle, Stefan Oehm, Michael Georg Hoesl, Christin Treiber-Kleinke, Lauri Peil, Mihaela Bozukova, Suki Albers, Abdul-Rahman Adamu Bukari, Torsten Semmler, Juri Rappsilber, Zoya Ignatova, Aleeza C. Gerstein, Nediljko Budiša
This paper uses adaptive laboratory evolution to replace tryptophan in E. coli with a synthetic analog, revealing that stress response inhibition, not translation rigidity, limits genetic code expansion.
Frontiers in Synthetic Biology · Aug 24, 2023Peer-reviewedSynthetic Biology