Stephen J. Goldfless

The paper analyzes TCR {beta} chain pairing in human T-cells to determine its role in CD4+ and CD8+ lineage specification, finding that {beta} chain pairs are more informative than individual chains for lineage prediction.

This study uses single-cell sequencing to analyze T cell receptor pairing, finding non-random β chain associations and public clone sharing, which reduce T cell repertoire diversity.

The paper develops a single-cell barcoding method to capture immune receptor sequences from tumor-infiltrating lymphocytes without sorting or culturing, demonstrating its application on ovarian cancer samples.