A Robust and Scalable Workflow for the Production of Circular Single-Stranded DNA for Genome Engineering Applications
Susi Mathews, Mahima Kapoor, Akschya Sivacoumar, Raj Acharya, Souvik Maiti, Debojyoti Chakraborty
This paper presents a scalable workflow for producing high-purity circular single-stranded DNA using an M13 phagemid system, achieving yields of 120-195 µg from 300 mL culture.
bioRxiv · Aug 17, 2026 · PreprintMolecular Biology