CRISPR/Cas9-Assisted Transformation-Efficient Reaction (CRATER), a novel method for selective transformation
L. J. Rothschild, D. T. Greenberg, J. R. Takahashi, K. A. Thompson, A. J. Maheshwari, R. E. Kent, G. McCutcheon, J. D. Shih, C. Calvet, T. D. Devlin, T. Ju, D. Kunin, E. Lieberman, T. Nguyen, F. G. Tran, , K. Fujishima
The paper introduces CRATER, a CRISPR/Cas9-based method that improves plasmid transformation efficiency by selectively cleaving unwanted byproducts, increasing efficiency from 20% to 97%.
bioRxiv · Sep 27, 2015 · PreprintSynthetic Biology