Efficient recovery of transgene-free edited tobacco using the transgene self-elimination system via de novo regeneration
Jianduo Zhang, Weiran Li, Jing Lv, Xinruitong Liu, 王萝萍, Yu Qian, Yuying Liu, Feiyan QI, Haoyi Xue, Xin Liu, Deng Lele, Fengjiao Wang, Jicang Xu, Kaili Xue, Xuepeng Liu, , Changbo Dai
This study develops a transgene self-elimination system in tobacco using CRISPR/Cas9 and BARNASE cassettes to generate transgene-free edited progeny through de novo regeneration.
bioRxiv · Oct 28, 2026 · PreprintMolecular Biology