Non-mechanical disruption of Bacillus subtilis spores allows sensitive and deep characterization of the minimal proteome for resuming a cellular lifestyle.
Yixuan Huang, Alphonse de Koster, Zhiwei Tu, Xiaowei Gao, Winfried Roseboom, Stanley Brul, Peter Setlow, Gertjan Kramer
This paper introduces a new sample preparation method, SPEED, which improves proteomic analysis of Bacillus subtilis spores by identifying more proteins and enabling differentiation between spore and vegetative cell proteins.
bioRxiv · Mar 8, 2024 · PreprintMicrobiology