Rapid and sensitive direct detection and identification of poliovirus from stool and environmental surveillance samples using nanopore sequencing
A. G. Shaw, M. Majumdar, C. Troman, A. O'Toole, B. Benny, D. Abraham, I. Praharaj, G. Kang, S. Sharif, M. M. Alam, S. Shaukat, M. Angez, A. Khurshid, N. Mahmood, Y. Arshad, , N. C. Grassly
This paper presents a nested PCR and nanopore sequencing protocol for rapid and sensitive detection and identification of poliovirus in stool and environmental samples, reducing the time compared to traditional cell-culture methods.
bioRxiv · Apr 27, 2020Microbiology